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目的:探讨外源性维生素C对缺氧诱导人滋养细胞凋亡的影响。方法:行实施人早孕期滋养细胞株TEV-1培养,经95%氮气处理30 min,行递增浓度的维生素C(0、1、5、50 ng/ml)继续孵育24 h后,各组细胞均应用噻唑兰(MTT)比色法、流式细胞术(FCM)法检测细胞增殖指数、凋亡率,同时利用黄嘌呤氧化酶法测定各组细胞超氧化物歧化酶(SOD)活性变化。结果:滋养细胞经缺氧诱导30 min后,与对照组相比,添加不同浓度组(1、5及50 ng/ml)维生素C对其增殖行为无明显影响。但维生素C具有增强滋养细胞SOD活性,减弱其凋亡程度的作用(P<0.05)。结论:维生素C能明显提高缺氧损伤滋养细胞的抗氧化能力,可能参与了妊娠的病理生理过程。
Objective: To investigate the effect of exogenous vitamin C on apoptosis of human trophoblast cells induced by hypoxia. Methods: The human gestational trophoblast cell line TEV-1 was cultured and treated with 95% nitrogen for 30 min. After continuous incubation with increasing concentrations of vitamin C (0, 1, 5 and 50 ng / ml) for 24 h, Cell proliferation index and apoptosis rate were detected by MTT colorimetric assay and flow cytometry (FCM). At the same time, the activity of superoxide dismutase (SOD) in each group was measured by xanthine oxidase method. Results: Compared with the control group, the addition of different concentration of vitamin C (1, 5 and 50 ng / ml) had no significant effect on the proliferation of nourishing cells induced by hypoxia for 30 min. However, vitamin C has the effect of enhancing SOD activity of trophoblast cells and attenuating the degree of apoptosis (P <0.05). Conclusion: Vitamin C can significantly increase the antioxidant capacity of trophoblast cells injured by hypoxia, which may be involved in the pathophysiology of pregnancy.