应用扫描电镜观察了经低温处理 (5℃ ,2 4h)和几丁质酶处理的棉铃虫围食膜的形态变化。结果表明 ,正常围食膜致密平滑 ,没有孔或缝。低温处理的围食膜产生了孔和缝 ,孔径可达15 0 nm,使核多角体病毒能进入中肠细胞。经几丁质酶处理的围食膜也出现孔和缝。处理时间越长 ,破坏越大。几丁质酶处理围食膜对蛋白质含量有一定影响 ,对糖含量变化的影响则较大 The morphological changes of the bollworm-treated peritrophic membrane were observed under scanning electron microscopy (SEM) at low temperature (5 ℃, 24 h) and chitinase treatment. The results showed that the normal peri-merous membrane dense and smooth, no holes or seams. Hypothermic peri-perforating membranes produce holes and seams with pore sizes up to 150 nm, allowing the nucleopolyhedrovirus to enter the midgut cells. Chitinase-treated peritoneal membranes also appear to have holes and seams. The longer the processing time, the greater the damage. Chitinase treatment of peritrophic membrane protein content has a certain impact on the changes in sugar content is greater