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目的研究微囊藻毒素-LR(MC-LR)对大鼠睾丸支持细胞中活性氧(ROS)水平和丙二醛(MDA)含量的影响。方法分离并培养10只健康18～20日龄清洁级雄性SD大鼠睾丸支持细胞。调整细胞密度为1×104～2×104个/ml,在终浓度分别为0(溶剂对照)、0.15、1.5、15μg/L的MC-LR培养液中染毒24h,采用MMT法测定细胞活性。调整细胞密度为2×105～4×105个/ml,在终浓度分别为0(溶剂对照)、0.15、1.5、15μg/L的MC-LR培养液中染毒6、12、24h,检测睾丸支持细胞中ROS和MDA含量。结果与溶剂对照组相比,染毒24h后各MC-LR染毒组细胞活性均有所增加,但差异均无统计学意义。与溶剂对照组比较,染毒6、24h时15μg/LMC-LR染毒组睾丸支持细胞ROS含量较高,差异均有统计学意义(P<0.05)。且随着MC-LR染毒剂量的增加和染毒时间的延长,睾丸支持细胞ROS含量呈上升趋势。不同染毒时间各染毒组睾丸支持细胞中MDA含量与溶剂组相比,差异无统计学意义。结论睾丸支持细胞暴露于本实验剂量MC-LR时可引起细胞中ROS含量升高,但不引起睾丸支持细胞MDA含量的改变。
Objective To investigate the effect of microcystin-LR (MC-LR) on reactive oxygen species (ROS) and malondialdehyde (MDA) content in rat testicular sertoli cells. Methods Ten testicular sertoli cells of healthy male Sprague-Dawley rats aged 18-20 days were isolated and cultured. The cell density was adjusted to 1 × 10 4 to 2 × 10 4 cells / ml, and the cell viability was measured by MTT assay in MC-LR medium with final concentrations of 0 (solvent control), 0.15, 1.5 and 15 μg / L, respectively. . The cell density was adjusted to 2 × 10 5 to 4 × 10 5 cells / ml, and the testis was detected in MC-LR medium with final concentration of 0 (solvent control), 0.15, 1.5 and 15 μg / L for 6, Supports ROS and MDA content in cells. Results Compared with the solvent control group, the activity of MC-LR cells increased after 24h exposure, but the difference was not statistically significant. Compared with the solvent control group, the content of ROS in testis supportive cells of 15μg / L MC-LR group was higher at 6 and 24 hours (P <0.05). With the increase of the dose of MC-LR and the prolongation of the exposure time, the content of ROS in the testicular supporting cells increased. Compared with the solvent group, there was no significant difference in the content of MDA in testis supporting cells in different exposure time groups. Conclusion The testicular sertoli cells exposed to the experimental dose of MC-LR can cause cells to increase ROS levels, but did not cause changes in testis support cells MDA content.