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目的观察通络药物参松养心胶囊提取干粉溶液对单个豚鼠心室肌细胞膜钠通道电流(I_(Na))、L 型钙通道电流(I_(Ca,L))的影响。方法用钙外液将参松养心胶囊干粉配制成不同浓度(1%、0.5%、0.25%)。用酶解法分离单个心室肌细胞,并用全细胞膜片钳记录技术记录电流。结果当药物浓度0.5%时,可以使 I_(Na)峰值电流密度从(27.2±5.4)pA/pF 降至(14.9±2.8)pA/pF,平均抑制率为44.8%±7.7%(n=5,P<0.05);药物浓度为1%,0.5%,0.25%时,分别对 I_(Ca,L)的峰值电流密度的抑制率为50.7%±5.6%,44.8%±6.5%,和19.2%±1.1%,(n=5,P<0.05)。药物可以使 I_(Na)、I_(Ca,L)的电流密度-电压曲线上移,但均不改变其激活、峰值和反转电位。结论参松养心胶囊提取干粉溶液对 I_(Na)、I_(Ca,L)具有阻滞作用,这可能是其抗心律失常和心肌保护作用药理机制的一部分。
Objective To observe the effects of Tongluo Drug Shensongyangxin capsule extract dry powder solution on sodium channel current (I_(Na)) and L type calcium channel current (I_(Ca,L)) of guinea pig ventricular myocytes. Methods The dry powder of Shensong Yangxin Capsule was formulated into different concentrations (1%, 0.5%, 0.25%) with calcium external fluid. Individual ventricular myocytes were isolated by enzymatic hydrolysis and currents were recorded using whole cell patch clamp recording techniques. Results When the drug concentration was 0.5%, the peak current density of I_(Na) could be reduced from (27.2±5.4) pA/pF to (14.9±2.8) pA/pF, and the average inhibition rate was 44.8%±7.7% (n=5). (P<0.05); When the concentration of drug was 1%, 0.5%, 0.25%, the peak current density of I_(Ca, L) was 50.7%±5.6%, 44.8%±6.5%, and 19.2%, respectively. ±1.1%, (n=5, P<0.05). The drug can shift the current density-voltage curve of I_(Na), I_(Ca, L) up, but does not change its activation, peak and reversal potential. Conclusion Shensongyangxin Capsule extract has a retarding effect on I_(Na) and I_(Ca, L), which may be part of the pharmacological mechanism of antiarrhythmic and myocardial protection.