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目的检测类风湿关节炎(rheumatoid arthritis,RA)患者外周血CD19~+CD24~(hi)CD38~(hi)调节性B细胞(regulatory B cells,Bregs)比例及功能的变化情况,初步探讨Bregs在RA发病中的作用。方法采用流式细胞染色方法检测健康对照组及RA患者外周血单核细胞(peripheral blood mononuclear cell,PBMC)中CD19~+CD24~(hi)CD38~(hi)B细胞和CD4 T细胞比例变化情况;活细胞荧光染料CFSE染色方法检测CD19~+CD24~(hi)CD38~(hi)B细胞对Nave CD4 T细胞增殖能力的影响;同时检测CD19~+CD24~(hi)CD38~(hi)B细胞分泌IL-10的能力及对CD4 T细胞分泌IFN-γ能力的影响。结果与健康对照组相比,RA患者外周血CD19~+CD24~(hi)CD38~(hi)B细胞比例显著降低,而CD4 T细胞比例显著升高,且随着病情严重程度升高,差异有统计学意义(P<0.05)。功能研究显示,RA患者外周血CD19+CD24hi CD38hiB细胞分泌IL-10水平显著降低(P<0.01),且其抑制Nave CD4 T细胞增殖及分泌IFN-γ的能力均显著降低(P<0.01)。结论 RA患者外周血CD19~+CD24~(hi)CD38~(hi)B细胞分泌IL-10能力下降,导致免疫抑制功能降低,进而引起CD4 T细胞比例升高和IFN-γ水平升高,影响RA病情进展。
Objective To detect the changes of the percentage and function of CD19 ~ + CD24 ~ (hi) CD38 ~ (h) regulatory B cells (Bregs) in peripheral blood of patients with rheumatoid arthritis (RA) The role of RA in the pathogenesis. Methods The proportion of CD19 ~ + CD24 ~ (hi) CD38 ~ (hi) B cells and CD4 T cells in peripheral blood mononuclear cells (PBMCs) of healthy controls and RA patients were detected by flow cytometry The effect of CD19 ~ + CD24 ~ (hi) CD38 ~ (hi) B cells on the proliferation of Nave CD4 T cells was detected by CFSE staining of living cells. The CD19 ~ + CD24 ~ (hi) CD38 ~ ) The ability of B cells to secrete IL-10 and their ability to secrete IFN-γ to CD4 T cells. Results Compared with the healthy controls, the percentage of CD19 ~ + CD24 ~ (hi) CD38 ~ (hi) B cells in peripheral blood of RA patients was significantly decreased while the proportion of CD4 T cells was significantly increased. As the severity of disease increased, There was statistical significance (P <0.05). The functional studies showed that the levels of IL-10 secreted by CD19 + CD24hi CD38hiB cells in peripheral blood of RA patients were significantly decreased (P <0.01), and the ability of inhibiting Nave CD4 T cell proliferation and IFN-γ secretion was significantly decreased (P <0.01 ). Conclusions The ability of IL-10 secreted by CD19 ~ + CD24 ~ (hi) CD38 ~ (hi) B cells in peripheral blood of RA patients is decreased, leading to the decrease of immunosuppressive function, which in turn leads to the increase of the proportion of CD4 T cells and the increase of IFN-γ RA disease progression.