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目的：研究卵巢浆、粘液性囊腺癌增殖细胞核抗原（ＰＣＮＡ）的表达及其临床病理意义。方法：应用ＰＣＮＡ的单克隆抗体ＰＣ１０，以ＡＢＣ免疫组织化学方法，对５０份卵巢浆液性囊腺癌、１８份卵巢粘液性囊腺癌和５份正常卵巢组织的石蜡标本作ＰＣＮＡ表达的检测，并根据其阳性细胞率评分。结果：卵巢浆液性囊腺癌ＰＣＮＡ阳性细胞率评分高于卵巢粘液性囊腺癌；组织学分化程度越低，ＰＣＮＡ阳性细胞率评分越高；临床分期越晚，ＰＣ－ＮＡ阳性细胞率评分越高；伴有淋巴转移者ＰＣＮＡ阳性细胞率评分高于无淋巴转移者；ＰＣＮＡ表达为强级的患者５ａ生存率明显低于ＰＣＮＡ表达为弱级者；差异均有显著性。结论：ＰＣＮＡ免疫组化检测可作为预测卵巢浆、粘液性囊腺癌淋巴转移和患者预后的重要参考指标。
Objective: To study the expression of proliferating cell nuclear antigen (PCNA) in ovarian pulp and mucinous cystadenocarcinoma and its clinicopathological significance. Methods: The expression of PCNA in 50 specimens of ovarian serous cystadenocarcinoma, 18 specimens of ovarian mucinous cystadenocarcinoma and 5 specimens of normal ovarian tissue was detected by ABC immunohistochemical method using PCNA monoclonal antibody PC10. And according to their positive cell rate score. Results: The positive rate of PCNA positive cells in ovarian serous cystadenocarcinoma was higher than that in ovarian mucinous cystadenocarcinoma. The lower the degree of histological differentiation was, the higher the positive rate of PCNA was. The later the clinical stage, the higher the score of PCNA positive cells The positive rate of PCNA with lymphatic metastasis was higher than that without lymphatic metastasis. The survival rate of 5a with PCNA was significantly lower than that with PCNA, the difference was significant. Conclusion: The immunohistochemical detection of PCNA can be used as an important reference index for predicting the lymph node metastasis of ovarian pulp and mucinous cystadenocarcinoma and the prognosis of patients.