过去的研究表明,大白鼠黑质多巴胺细胞在完成最后一次细胞分裂后即开始细胞形态的分化,与此同时,以特异神经递质为特征的化学分化也开始出现.细胞形态分化和化学分化的进行可能与细胞间的相互作用有一定关系.本工作采用酪氨酸羟化酶(TH)抗体的免疫细胞化学结合电镜方法,研究了胚胎黑质多巴胺细胞与周围细胞之间的接触.材料用13—14天胚胎大白鼠,经4％多聚甲醛、0.5％戊二醛的固定液 Previous studies have shown that dopamine neurons in rat substantia nigra begin to differentiate into cells after the last cell division, and at the same time, chemical differentiation characterized by specific neurotransmitter has begun to appear. Morphological differentiation and chemical differentiation May have a certain relationship with the cell-cell interactions.In this work, the use of tyrosine hydroxylase (TH) antibody immunocytochemistry combined with electron microscopy methods to study the contact between embryonic nigra dopamine cells and the surrounding cells. 13-14 days embryos in rats, 4% paraformaldehyde, 0.5% glutaraldehyde fixative