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为了探究外源NO对半夏体内次生代谢的调控机制,以半夏组培苗为材料,研究在喷洒不同浓度SNP(NO供体)后体内苯丙氨酸解氨酶(PAL)活性、谷氨酸合酶(GOGAT)活性、谷草转氨酶(GOT)活性、总生物碱质量分数和鸟苷质量分数的变化和规律.结果表明:不同浓度SNP均能提高半夏组培苗体内苯丙氨酸解氨酶(PAL)、谷氨酸合酶(GOGAT)、谷草转氨酶(GOT)活性,增加次生代谢产物总生物碱和鸟苷质量分数.其中,1mmol/L SNP处理组在第24天时效果最好,总生物碱及鸟苷质量分数分别比对照增加了66.1%,188.3%.外源NO可能通过提高半夏组培苗体内PAL,GOGAT,GOT活性,加速无机氮的同化及转化效率,提供更多的代谢前体物以促进总生物碱及鸟苷积累.
In order to explore the regulatory mechanism of exogenous NO on the secondary metabolism in Pinellia ternata, the tissue culture of Pinellia ternata was used to study the activity of phenylalanine ammonia-lyase (PAL) in vivo after spraying different concentrations of SNP (NO donor) Glutamic acid synthase (GOGAT) activity, glutamic oxaloacetic transaminase (GOT) activity, total alkaloid mass fraction and guanosine mass fraction.The results showed that different concentrations of SNP can improve the tissue culture in vivo Pinellia (PAL), glutamic acid synthase (GOGAT) and aspartate aminotransferase (GOT), and increased the total alkaloids and guanosine content of secondary metabolites.Among them, The best total alkaloids and guanosine were 66.1% and 188.3% higher than the control respectively.Exogenous NO could accelerate the assimilation and transformation efficiency of inorganic nitrogen by improving the activities of PAL, GOGAT and GOT in the tissue culture seedlings of Pinellia ternate , To provide more metabolic precursors to promote the accumulation of total alkaloids and guanosine.