Chemical compositions and microsturcture of mature human tooth enamel were investigated by XRD , FTIR and SEM to further understand the characteristics of tooth enamel. In order to obtain apatite crystals chemically and structurally similar to those in tooth enamel, biomimetic way was employed. Selfassembled monolyers terminated with-SO3 H groups were used as deposition substrates and 1.5 SBF ( the concentrations of Ca2+ and PO43- ions 1.5 times than those in simulated body fluid ) with and without 5 ppm F- were used as soaking medium. The XRD and FTIR results showed that both the deposited fluoride-substituted hydroxyapatite( F-HAp ) crystals in 1.5 SBF with F- and hydroxyapatite ( HAp ) crystals in 1.5 SBF were carbonate-containing, mimicking human tooth enamel in chemical compositions. The SEM photos showed that needle-like F-HAp crystals bad large aspect ratios and grew in bundles, which were similar to the crystals in human tooth enamel.The results provide available information on dental restoration.