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为评价蔬菜灰霉病菌对啶酰菌胺的抗性风险,采用紫外线照射和药剂驯化相结合的诱导方法,对蔬菜灰霉病菌D9、L17、J9亲本敏感菌株进行了室内啶酰菌胺抗性诱导,并采用菌落直径法比较了抗、感菌株在生物学性状方面的差异。结果显示,共获得不同抗性水平的突变体23株,其中突变体D9-4的抗性倍数为253.76倍,达高抗水平。突变体菌丝生长速率较敏感菌株有所下降,抗、感菌株的适宜生长温度均为25℃,敏感菌株最适pH为5,突变体最适pH为7,突变体适应酸碱度和温度变化的能力较敏感菌株差;低抗突变体产孢量和孢子萌发率与敏感菌株无显著差异,但高抗突变体产孢量和孢子萌发率较敏感菌株显著降低;在无药条件下,敏感菌株致病力强于抗性突变体,而在药剂胁迫下,突变体表现出一定的耐药性,致病力强于敏感菌株;6株突变体无药继代培养15代后,突变体抗性稳定遗传。此外,蔬菜灰霉病菌对啶酰菌胺与其它5种杀菌剂之间没有交互抗性。研究表明,蔬菜灰霉病菌抗啶酰菌胺突变体适合度较低,但在药剂选择压力下容易形成啶酰菌胺抗性群体,在生产中需引起注意。
In order to evaluate the resistance risk of Botrytis cinerea to boscalid, UV-irradiation and chemical domestication were used to induce the boscalid resistance in Botrytis cinerea isolates D9, L17 and J9 Induced, and colony diameter method was used to compare the biological characteristics of the resistant and susceptible strains. The results showed that a total of 23 strains with different resistance levels were obtained, in which the resistance multiple of mutant D9-4 was 253.76 times, reaching a high level of resistance. The growth rate of mutant hyphae was lower than that of the susceptible strain. The optimal growth temperature of the resistant and susceptible strains was 25 ℃, the optimum pH of the sensitive strain was 5, the optimum pH of the mutant was 7, and the mutants were adapted to pH and temperature The results showed that the spore germination rate and spore germination rate of the low resistant mutants were not significantly different from those of the susceptible strains, but the spore germination rate and spore germination rate of the high resistant mutants were significantly lower than those of the susceptible strains. In the absence of drug, the susceptible strains The pathogenicity was stronger than that of the resistant mutants. However, under the drug stress, the mutants showed some resistance and the pathogenicity was stronger than that of the susceptible strains. After six generations of mutants were subcultured for 15 generations, Sexually stable inheritance. In addition, Botrytis cinerea had no cross-resistance to boscalid and five other fungicides. Studies have shown that Botrytis cinerea boscalid mutants low fitness, but easy to form under the pressure of drug selection boscalid resistant population, need to pay attention in the production.