本文采用丹酸氯试剂作为往前衍生剂，用反相高效波相色谱法检测胸水中多胺的浓度。实验条件：荧光激发波长340nm，发射波长515nm。流动相为甲醇一水。本法测得多肤的回收率为：腐胶93％～107％，精味89％～103％，精胺98％～105％．变异系数分别为：腐胶7．4％～9．5％，精脒43％～6．玉％，精胺3．3％～4．2％。方法稳定、可靠． In this article, the dilute citrate chloride reagent was used as a derivatization agent, and the concentration of polyamines in the pleural fluid was detected by reversed phase high performance wave chromatography. Experimental conditions: fluorescence excitation wavelength 340 nm, emission wavelength 515 nm. The mobile phase is methanol-water. This method measured much of the skin’s recovery rate: 93% to 107% of fermented rubber, fine taste 89% to 103%, spermine 98% to 105%. The coefficients of variation were: 7.4%-9.5% for rotting and 43%-6 for fine. Jade%, spermine 3.3% to 4.2%. The method is stable and reliable.