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利用纯化的Ｂｔ毒素蛋白（６７ｋＤａ）作为标准蛋白和免疫抗原，建立了检测转基因抗虫棉植株内Ｂｔ毒蛋白含量的抗体夹心ＥＬＩＳＡ方法。检测极限１μｇ·Ｌ－ １，线性范围１－ １５μｇ·Ｌ－ １，板内误差Ｃ．Ｖ．＜ ５％ ，板间误差Ｃ．Ｖ．＜ ５％ ，平均样品回收率９４．９４％ 。经稀释度试验等质量控制试验，表明所建立的ＥＬＩＳＡ方法是可靠的，可用于转基因棉花植株中Ｂｔ毒素蛋白含量的测定。
Using purified Bt toxin protein (67 kDa) as a standard protein and immunogen, a sandwich ELISA method was developed for the determination of Bt toxin content in transgenic Bt cotton plants. Detection limit 1μg · L- 1, the linear range of 1- 15μg · L- 1, intraplate error C. V. <5%, the error between the plates C. V. <5%, the average sample recovery 94.94%. The quality control test such as dilution test showed that the established ELISA method is reliable and can be used for the determination of Bt toxin protein content in transgenic cotton plants.