为探索斜纹夜蛾蛹畸形发育的生理机制,在室内将斜纹夜蛾幼虫饲养至蛹期,测定了斜纹夜蛾蛹的生物学参数,并采用双向电泳技术对蛹整体可溶蛋白质进行分析。结果表明:畸形蛹率达(18.8±1.2)%,其畸形蛹的发育历期明显短于正常蛹,为(9.5±0.7)d(P<0.01)。畸形蛹中雌蛹和雄蛹的体重也明显小于正常蛹(P<0.01)。在正常蛹中共检测到约147个蛋白质点,主要集中在分子量1.1×104至7.6×104、等电点3.23至9.80之间。在畸形蛹中共检测到149个蛋白质点,主要集中在分子量1.0×104至7.7×104、等电点3.61至9.61之间。在正常蛹中有45个特异表达蛋白质点,畸形蛹中有47个特异表达蛋白质点。 In order to explore the physiological mechanism of development of pupal deformity in Spodoptera litura, the larva of Spodoptera litura was fed to the pupal stage indoors, the biological parameters of the pupae of Spodoptera litura were determined, and the total soluble proteins of pupae were analyzed by two-dimensional electrophoresis. The results showed that the malformation pupa rate was (18.8 ± 1.2)%, and the developmental duration of malformed pupae was significantly shorter than that of normal pupae (9.5 ± 0.7) d (P <0.01). The body pupal and male pupal weight of deformity pupae were also significantly lower than normal pupae (P <0.01). A total of 147 protein spots were detected in normal pupae, mainly concentrated in the molecular weight of 1.1 × 104 to 7.6 × 104, isoelectric point of 3.23 to 9.80. A total of 149 protein spots were detected in deformity pupa, which were mainly concentrated in the molecular weight of 1.0 × 104 to 7.7 × 104 and isoelectric point of 3.61 to 9.61. There are 45 protein spots specifically expressed in normal pupae and 47 specifically expressed protein spots in malformed pupae.