论文部分内容阅读
目的探讨超顺磁性氧化铁微粒(SPIO)体外标记骨髓间充质干细胞(BMSCs)及体内示踪移植入大鼠心肌梗死心脏的BMSCs的能力。方法使用左旋多聚赖氨酸-SPIO共培养方式标记BMSCs。采用普鲁士蓝染色观察细胞内铁颗粒,流式细胞术检测细胞活力,将经过SPIO标记的干细胞移植入心肌梗死大鼠心脏,应用1.5TMRI系统行磁标记干细胞成像。超声心动图检测各组心脏的左室射血分数(EF),左室舒张末期内径(LVIDd),左室收缩末期内径(LVIDs)及短轴缩短率(FS)。结果普鲁士蓝染色显示,SPIO标记的BMSCs细胞胞质内出现细小的蓝色铁颗粒,标记效率为(99.81±1.57)%;与正常未标记细胞相比较,细胞的活力差异无统计学意义(P>0.05)。标记了SPIO的BMSCs体内MRI成像时显示,细胞移植区域信号缺失,对应区域病理切片普鲁士蓝染色可见胞浆内染色阳性的细胞。超声心动图显示,PBS组FS移植前后没有明显变化,BMSC组FS从移植前的(23.1±1.88)%上升到第1周的(31.28±4.15)%。BMSC组EF在移植前是(51.13±5.07)%,第1周时上升到(60.12±8.40)%。结论 SPIO能成功地标记BMSCs,且对BMSCs的活力无明显影响。BMSCs移植后能改善心梗大鼠的心功能。SPIO标记的BMSCs移植后在大鼠体内的分布、迁移过程可用MRI进行检测评价。
Objective To investigate the ability of SPIO-labeled bone marrow mesenchymal stem cells (BMSCs) and BMSCs traced in vivo to myocardial infarction in vivo. Methods BMSCs were labeled with L-polylysine-SPIO co-culture. The intracellular iron particles were observed by Prussian blue staining. The viability of the cells was detected by flow cytometry. The SPIO-labeled stem cells were transplanted into the heart of myocardial infarction rats. The magnetic-labeled stem cells were imaged by 1.5T MRI system. Cardiac left ventricular ejection fraction (EF), left ventricular end diastolic dimension (LVIDd), left ventricular end-systolic dimension (LVIDs) and short axis shortening (FS) were measured by echocardiography. Results Prussian blue staining showed that the SPIO-labeled BMSCs showed fine blue iron particles in the cytoplasm, with a labeling efficiency of (99.81 ± 1.57)%. Compared with normal unlabeled cells, there was no significant difference in the viability of cells (P > 0.05). Marked the SPIO of BMSCs in vivo MRI imaging, cell transplantation area signal is missing, corresponding to the regional pathology Prussian blue staining shows cytoplasm staining positive cells. Echocardiography showed no significant changes before and after FS transplantation in PBS group. FS in BMSC group increased from 23.1 ± 1.88% before transplantation to 31.28 ± 4.15% in the first week. EF in BMSC group was (51.13 ± 5.07)% before transplantation and increased to (60.12 ± 8.40)% in the first week. Conclusion SPIO can successfully label BMSCs without any significant effect on the viability of BMSCs. BMSCs transplantation can improve cardiac function in myocardial infarction rats. SPIO-labeled BMSCs after transplantation in vivo distribution in rats, the migration process can be evaluated by MRI.