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研究临床乳腺癌标本中细胞凋亡的发生情况，评价其在预测后在的意义。方法搜集９１例浸润性乳腺癌的石蜡组织切片，运用原位ＤＮＡ断裂位点的末端标记法，检测细胞凋亡，计算凋亡细胞占肿瘤细胞的百分比，求得凋亡指数。结果本组病例细胞凋亡发生率为９１．２％，凋亡指数分为两组：０～０．２１和０．２８～０．６２，凋亡指数高低与腋淋巴结转称相关（Ｐ＜０．０１）。生存率单因素分析中，凋亡指数高的病例，无病生存率（Ｐ＝０．００９５）及总生存率（Ｐ＝０．０３４８）均优于凋亡指数低的病例。但Ｃｏｘ模型多因素分析末能指示凋亡指数是一个独立的预后指标。结论在乳腺癌组织中，细胞凋亡是一种自发现象，其发生情况各有不同，研究初步提示了细胞凋亡在乳腺癌预后中的作用，了解了凋亡和腋淋巴结转移的相关性。
To study the occurrence of apoptosis in clinical breast cancer specimens and evaluate its significance after prediction. Methods Paraffin sections of 91 cases of invasive breast cancer were collected. The end-labeling method of in situ DNA fragmentation was used to detect apoptosis. The percentage of apoptotic cells in tumor cells was calculated and apoptosis index was obtained. Results The incidence of apoptosis in this group was 91.2%. The apoptotic index was divided into two groups: 0-0.21 and 0.28-0.62. The level of apoptotic index was related to the axillary lymph node turnover (P< 0.01). In the single-factor survival analysis, cases with high apoptotic index, disease-free survival (P=0.0095) and overall survival (P=0.0348) were better than those with low apoptotic index. However, the multivariate analysis of the Cox model can indicate that the apoptotic index is an independent prognostic indicator. Conclusion In breast cancer tissues, apoptosis is a self-discovery and its occurrence is different. The study tentatively suggests the role of apoptosis in the prognosis of breast cancer and the relationship between apoptosis and axillary lymph node metastasis.