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目的 探讨hTR、hTERT与端粒酶活性的表达是否与肺癌的发生发展有关 ,深入了解端粒酶基因对端粒酶活性的调控是在基因水平还是在转录水平。方法 采用TRAP PCR和RT PCR方法分别检测 6 8例肺癌组织、6 8例癌旁组织端粒酶活性、端粒酶基因hTR和hTERT的表达。结果 6 8例肺癌组织中端粒酶阳性率为 79%,HTR阳性率为 98 5 %,HTERT阳性率为 91 2 %。 6 8例癌旁组织中 ,无一例表达端粒酶阳性 ,且大多数癌旁组织均表达HTR(91 2 %) ,而HTERT在 6 8例癌旁组织中仅 7例为阳性。结论 肺癌组织中表达高频率的端粒酶活性而癌旁组织无一例表达端粒酶阳性说明了端粒酶的活性是肺癌发生发展所必须的。与HTR相比 ,HTERT同端粒酶具有更高的一致性 ,其一致率为 88 9%。HTR与HTERT可能是在转录后或翻译后水平对端粒酶进行调控的。
Objective To investigate whether the expression of hTR, hTERT and telomerase activity is related to the occurrence and development of lung cancer, and to find out whether telomerase activity is regulated at the gene level or the transcription level. Methods The telomerase activity, telomerase gene hTR and hTERT expression in 68 lung cancer tissues and 68 para-cancerous tissues were detected by TRAP PCR and RT-PCR respectively. Results The positive rate of telomerase in 68 cases of lung cancer was 79%, the positive rate of HTR was 98.5% and the positive rate of HTERT was 91.2%. None of the 68 adjacent tissues showed telomerase positive expression, and most of the adjacent tissues expressed HTR (91.2%), while only 7 of 68 adjacent tissues showed positive staining. Conclusion The high frequency of telomerase activity in lung cancer tissues and the positive expression of telomerase in paracancer tissues indicate that telomerase activity is necessary for the development of lung cancer. Compared with HTR, HTERT had higher homology with telomerase with a concordance rate of 88.9%. HTR and HTERT may regulate telomerase at post-transcriptional or post-translational levels.