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目的:探讨尿视黄醇结合蛋白(RBP)、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、尿胱蛋白酶抑制剂C(Cystatin C)的测定对原发性高血压早期肾损害诊断的敏感性及临床意义。方法:选择2011年4月～2011年11月在新疆自治区人民医院肾病科住院的原发性高血压患者69例作为观察组,另设健康体检者30例为对照组。尿RBP、Cystatin C测定采用酶联免疫法(ELISA)检测,尿NAG测定采用对硝基苯酚(PNP)比色法检测。结果:观察组中尿RBP、NAG、Cystatin C的测定含量明显高于正常对照组(P<0.001),通过ROC曲线、诊断试验结果显示:尿RBP的曲线下面积为0.962,95%可信区间为0.921～0.983,较尿NAG、Cystatin C更具有敏感性(P<0.001)。结论:检测尿RBP有助于高血压肾损害的早期监测,对预防高血压性肾病的发生、发展具有重要的临床价值。
Objective: To investigate the effects of urinary retinol binding protein (RBP), urinary N-acetyl-β-D-glucosaminidase (NAG) and cystatin C on essential hypertension early renal Sensitivity and clinical significance of damage diagnosis. Methods: From April 2011 to November 2011, 69 patients with essential hypertension who were hospitalized in Department of Nephrology, People’s Hospital of Xinjiang Uygur Autonomous Region were selected as the observation group. Another 30 healthy subjects were selected as the control group. Urine RBP and Cystatin C were detected by enzyme-linked immunosorbent assay (ELISA) and urinary NAG assay by p-nitrophenol (PNP) assay. Results: The contents of urine RBP, NAG and Cystatin C in the observation group were significantly higher than those in the normal control group (P <0.001). According to the ROC curve, the diagnostic test results showed that the area under the curve of urinary RBP was 0.962 and 95% confidence interval 0.921 ~ 0.983, more sensitive than urine NAG and Cystatin C (P <0.001). Conclusion: The detection of urine RBP contributes to the early monitoring of hypertensive renal damage, and has important clinical value in the prevention and treatment of hypertensive nephropathy.