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目的:研究应用平行绑定多电极细胞外记录技术探讨海人藻酸(KA)注射毁损Meynert基底核(NBM)后海马CA1区自发放电活动的改变。方法:雄性SD大鼠在水合氯醛麻醉和脑立体定位仪引导下,KA注射后破坏双侧NBM,一周后用改装的平行绑定多电极记录大鼠海马CA1区自发放电活动。结果:(1)与传统的细胞外单电极或多电极记录相比,本方法电极制备简单、灵活、造价低廉,细胞损伤小,可同时记录单个核团内多个神经元或相邻脑区多个神经元的活动,便于进一步对神经元环路活动进行分析。结合锋电位分类技术,可对单一通道获得的多个神经元活动进行甑别,大大提高实验精度和效率;(2)比较NBM毁损组和对照组核团放电发现:NBM毁损组大鼠CA1区自发放电频率明显减少,其中单个放电与爆发式(burst)放电类型的平均放电频率同时降低;NBM毁损组自发放电类型发生改变,burst数量增加,但burst内发放频率下降,burst间隔延长。结论:(1)平行绑定多电极技术简便、易行、灵活,结合多通道记录技术可为开展神经元环路活动研究提供有力工具;(2)NBM毁损可致大鼠海马CA1区自发放电频率减少和放电模式改变,提示NBM胆碱能系统参与海马环路的神经活动调控,NBM损伤所致海马自发放电活动的改变可能有助于解释阿尔茨海默病认知功能的下降。
OBJECTIVE: To study the changes of spontaneous discharge activity in the hippocampal CA1 region after KA injection of Meynert’s nucleus (NBM) by parallel-binding multi-electrode extracellular recording technique. Methods: Male SD rats were killed by bilateral injection of KA under the guidance of chloral hydrate anesthesia and stereotactic apparatus. One week later, spontaneous discharge activities of hippocampal CA1 region of rats were recorded by modified parallel binding multi-electrode. Results: (1) Compared with traditional extracellular single electrode or multi-electrode recording, the method has the advantages of simple and flexible electrode preparation, low cost and small cell damage, and can simultaneously record multiple neurons or adjacent brain regions in a single nucleus The activity of multiple neurons facilitates further analysis of neuronal loop activity. Combining with the technique of front potential classification, multiple neuronal activities obtained from a single channel can be distinguished from each other, which greatly improves the accuracy and efficiency of the experiment. (2) Compared with the NBM group and control group, The frequency of spontaneous discharge decreased obviously. The average discharge frequency of single discharge and burst discharge decreased at the same time. The type of spontaneous discharge in NBM damaged group changed and the number of burst increased, but the burst frequency decreased and burst interval prolonged. Conclusions: (1) Parallel binding multi-electrode technique is simple, easy and flexible, and combined with multi-channel recording technology can provide a powerful tool for the study of neuronal loop activity; (2) NBM damage can cause spontaneous discharge in rat hippocampal CA1 area Changes in frequency and pattern of discharge suggest that NBM cholinergic system is involved in the regulation of neural activity in the hippocampal loop. Changes in spontaneous discharge activity of hippocampus caused by NBM injury may help to explain the decline of cognitive function in Alzheimer’s disease.