用乙型肝炎病毒(HBV)DNA转染的2215细胞株,对大黄挥发油体外抗HBV作用进行研究。同时应用四甲基偶氮唑盐(MTT)比色法检测药物对细胞的毒性。选用阿昔洛韦(无环鸟苷)作阳性对照药。结果发现大黄挥发油对细胞的半数毒性浓度(CD_(50))>0.125 g/L,其浓度在0.0625g/L以下时,细胞存活率在90％以上,对HBsAg和HBeAg的最大抑制率分别是70.71±5.4％和30.99±5.3％,表明大黄挥发油具有体外抗HBV作用。 The 2215 cell line transfected with hepatitis B virus (HBV) DNA was used to study the anti-HBV effect of rhubarb volatile oil in vitro. At the same time, tetrazolium (MTT) colorimetry was used to detect the toxicity of the drug to the cells. Acyclovir (acyclovir) was used as a positive control drug. The results showed that the cytotoxic concentration of rhubarb volatile oil on cells was more than 0.125 g/L, and when the concentration was below 0.0625 g/L, the cell viability was over 90%. The maximum inhibition rates of HBsAg and HBeAg were 70.71±5.4% and 30.99±5.3%, indicating that rhubarb volatile oil has anti-HBV effect in vitro.