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目的:分析辽宁省手足口病(Hand-Foot-Mouth Disease,HFMD)肠道病毒71型分离株(Enterovirus 71,EV71)的分子特征。方法:从2008年和2009年手足口患者标本中分离EV71病毒,随机挑取18株分离株经RT-PCR扩增VP1区,并进行核苷酸序列分析。结果:18株EV71与C4亚型代表株具有较高的核苷酸序列同源性,在系统进化树上,其与C4亚型代表株处于同一分支,属于C4基因亚型。结论:2008年和2009年辽宁省HFMD的主要流行株为EV71 C4亚型,在进化树中形成紧密相连的二簇,处于不同的进化链中,提示辽宁省可能存在多个传播链。
Objective: To analyze the molecular characteristics of enterovirus 71 (EV71) in Hand-Foot-Mouth Disease (HFMD) in Liaoning Province. Methods: EV71 virus was isolated from specimens of hand, foot and mouth in 2008 and 2009, and 18 isolates were randomly selected to amplify the VP1 region by RT-PCR. Nucleotide sequence analysis was performed. Results: The 18 representative strains of EV71 and C4 subtypes had high nucleotide sequence homology. On the phylogenetic tree, they were in the same branch with the C4 subtype representative strain, belonging to C4 subtype. Conclusion: The major epidemic strains of HFMD in Liaoning Province in 2008 and 2009 are EV71 C4 subtypes, forming two closely related clusters in the evolutionary tree, which are in different evolutionary chains, indicating that there may be multiple propagation chains in Liaoning Province.