为观察培养的神经干细胞克隆球内部的超微结构特征,采用无血清培养技术,在体外进行小鼠纹状体神经干细胞克隆球的培养传代,经过免疫细胞化学鉴定后,对单一的神经干细胞克隆球进行固定,常规透射电镜观察。结果表明,神经干细胞可以在bFGF等生长因子存在的情况下,在无血清培养液内增殖生成悬浮状态的神经干细胞克隆球,这种克隆可被诱导生成神经细胞和神经胶质细胞,电镜下,神经干细胞克隆球内部细胞相互间可形成特化的膜性结构,细胞内可有小泡出现,部分细胞有凋亡的形态。 In order to observe the ultrastructural characteristics of the spheres of cultured neural stem cells, serum-free culture techniques were used to culture and subculture the mouse NSCs in vitro. After identification by immunocytochemistry, single neural stem cell clone Ball fixed, conventional transmission electron microscopy. The results showed that neural stem cells can proliferate into serum-free culture medium in the presence of growth factors such as bFGF to generate a suspension of neural stem cells in suspension, which can be induced to produce neural cells and glial cells. Under electron microscope, Neural stem cell cloning ball within the cells can form a special membrane structure, the cells may have vesicles, some of the cells in the form of apoptosis.