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目的:研究血管内皮生长因子(VEGF)、p53蛋白、微血管密度(MVD)在非小细胞肺癌中的表达及其相关性,探讨其在非小细胞肺癌发生、发展中的作用。方法:采用免疫组织化学S-P法检测80例非小细胞肺癌组织、20例肺良性病变组织中VEGF、p53蛋白的表达,并对肺癌组织中CD34单抗标记的血管计数MVD。结果:肺癌组织VEGF、p53蛋白阳性表达率明显高于肺良性病变组织(P<0.05)。VEGF、p53蛋白表达与肺癌患者的年龄、性别、组织学类型均无关(P>0.05),与肺癌组织的分化程度、TNM分期及淋巴结转移均有关(P<0.05)。VEGF与p53蛋白表达呈正相关(P<0.05)。在肺癌组织中,VEGF、p53蛋白表达阳性组MVD分别明显高于VEGF、p53蛋白表达阴性组(P<0.05);p53蛋白与VEGF表达均阳性组MVD明显高于p53蛋白与VEGF表达均阴性组(P<0.05)。结论:VEGF、p53可能与肺癌的发生、发展有关。VEGF与p53蛋白在肺癌组织中的表达呈正相关,提示突变型p53基因可能上调VEGF的表达。肺癌组织中MVD与VEGF、p53蛋白表达密切相关,突变型p53基因和VEGF在肺癌血管形成中具有协同作用。
Objective: To study the expression and correlation of vascular endothelial growth factor (VEGF), p53 protein and microvessel density (MVD) in non-small cell lung cancer (NSCLC) and to explore its role in the development and progression of non-small cell lung cancer. Methods: Immunohistochemical S-P method was used to detect the expression of VEGF and p53 protein in 80 cases of non-small cell lung cancer and 20 cases of benign lung disease. The counts of MVD in CD34 monoclonal antibody in lung cancer were calculated. Results: The positive rates of VEGF and p53 in lung cancer tissues were significantly higher than those in benign lung tissues (P <0.05). The expression of VEGF and p53 had no correlation with the age, sex and histological type (P> 0.05) of patients with lung cancer, which was correlated with the degree of differentiation, TNM stage and lymph node metastasis (P <0.05). VEGF and p53 protein expression was positively correlated (P <0.05). In lung cancer, the MVD of VEGF and p53 protein positive group was significantly higher than that of VEGF and p53 protein expression (P <0.05); MVD of p53 protein and VEGF positive group was significantly higher than that of p53 protein and VEGF negative group (P <0.05). Conclusion: VEGF and p53 may be related to the occurrence and development of lung cancer. The positive correlation between VEGF and p53 protein expression in lung cancer suggests that mutant p53 may up-regulate the expression of VEGF. MVD in lung cancer is closely related to the expression of VEGF and p53, and the mutant p53 gene and VEGF have a synergistic effect in angiogenesis of lung cancer.