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对引起牙鲆体表溃烂的病原纤毛虫—指状拟舟虫诱导牙鲆免疫反应产生的免疫球蛋白IgM进行分析 ,结果表明 ,病原纤毛虫免疫注射牙鲆 ,可诱导牙鲆发生特异性免疫反应 ,产生抗体。牙鲆抗指状拟舟虫血清的凝集试验 ,发现纤毛虫停止游动并发生虫体聚集 ;抗血清经与标准分子量和鼠IgM单克隆抗体以及对照血清的SDS -PAGE电泳比较分析证明 ,牙鲆抗指状拟舟虫血清免疫球蛋白IgM重链分子量为 710 0 0 ,轻链分子量为 2 30 0 0 ;IgM为四聚体 ,分子量为 752 0 0 0。
The result of immunoglobulin IgM induced by the immune response of Paralichthys olivaceus induced by the surface fester of flounder Paralichthys olivaceus showed that immunization of Japanese flounder with the pathogen ciliates could induce the specific immunity of Japanese flounder Reaction, produce antibodies. Paralichthys olivaceus agglutination test finger carnivore test found ciliates stop swimming and parasites aggregation; antiserum by standard molecular weight and murine IgM monoclonal antibody and control serum by SDS-PAGE electrophoresis analysis shows that teeth The IgM heavy chain of Paralichthys olivaceus serum immunoglobulin is 710 0 0, the light chain molecular weight is 2 30 0 0, the IgM is tetramer and the molecular weight is 752 0 0 0.