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目的动态研究中药复方制剂抑瘤饮体内对小鼠S180移植肿瘤组织中Bcl-2和Bax表达的影响。方法BALB/c小鼠右腋皮下接种S180肿瘤细胞24 h后开始,抑瘤饮组小鼠每日灌服抑瘤饮,对照组小鼠每日灌服等量凉开水。分别于第10、20、30、40 d杀鼠取瘤制成切片,免疫组织化学染色法检测肿瘤组织中Bcl-2和Bax表达,以图像分析系统对染色结果进行测定。结果各时间点抑瘤饮组肿瘤组织中Bcl-2表达均低于对照组,Bax表达均高于对照组。结论抑瘤饮体内抑瘤作用可能与下调肿瘤组织中Bcl-2表达,上调肿瘤组织中Bax表达,促进肿瘤细胞凋亡有关。
Objective To investigate the effect of Yiliuyin Decoction (TCM compound prescription) on the expression of Bcl-2 and Bax in S180 tumor in mice. Methods The right axilla of BALB / c mice were inoculated subcutaneously with S180 tumor cells for 24 hours. The mice in Yirui Yin group were fed with Yiliuyin once a day, and the control mice were orally given the same amount of cold water daily. The mice were sacrificed on the 10th, 20th, 30th and 40th days respectively to make slices. The expression of Bcl-2 and Bax in the tumor tissues was detected by immunohistochemical staining. The staining results were determined by image analysis system. Results The expression of Bcl-2 in tumor-bearing yi-jun-drink group was lower than that in control group at each time point, and the expression of Bax was higher than that in control group. Conclusion The antitumor effect of Yiliuyin Decoction may be related to down-regulating the expression of Bcl-2, up-regulating the expression of Bax in tumor tissue and promoting the apoptosis of tumor cells.