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虽然分析血脂肪酸的方法学文章较多。但测定的样本量较小,难以评价年龄、性别等因素的影响以及提出正常参考值。本文通过240名南京地区健康成人脂肪酸谱的测定,分析性别、年龄因素对血脂肪酸含量的影响。研究对象系南京市一家工厂的健康成年职工。其中20～29岁、30～39岁、40～49岁、50～59岁四个年龄组各60人,每年龄组男女各30人,统一采肘静脉血分离血清备检。检测方法采用毛细管气相色谱法,仪器为 HP6890 Series 气相色谱仪。脂肪酸标准及有关试剂为德国公司产品。血清脂肪酸测定工作主要在德国 Ulm 大学临床化学研究所完成。检验分析结果表明,健康成人血中主要的脂肪酸按其含量多少,游离脂肪酸依此为:C18:1、C16:0、C18:2、C18:0、C20:4、C20:0、C22:6、C14:0;总脂肪酸依次可排列为:C18:2、C16:0、C18:1、C18:0、C20:4、C22 6、C20:0、C14:0。其中游离脂肪酸与总脂肪酸含量的比值约为1:7;游离脂肪酸中饱和脂肪酸与不饱和脂肪酸含量的比值为1:1.5,而在总脂防酸中该比值为1:1.9,说明总脂肪酸中不饱和脂肪酸含量明显升高。女性健康成人8种血清总脂肪酸的含量明显高于男性,除 C20:0、C20:4之外,均有显著性的差异(P<0.05)。而游离脂肪酸在男、女健康成人中未见显著性差异(P>0.05)。健康成人8种游离脂肪酸中,油酸(C18:1)随着年龄的增加而增加,其中50-59岁年龄组含量最高,而且与其它年龄组相比具有显著性(P<0.05)。
Although there are many methodologies for analyzing blood fatty acids. However, the sample size is small, it is difficult to evaluate the impact of age, gender and other factors and to provide normal reference values. This article analyzes the fatty acid profiles of healthy adults in Nanjing, and analyzes the effects of gender and age on blood fatty acid content. The research object is a healthy adult worker in a factory in Nanjing. Among them, there were 60 age groups of 20 to 29 years, 30 to 39 years, 40 to 49 years, and 50 to 59 years. Each age group consisted of 30 men and women. The elbow venous blood was collected and collected for serum preparation. The detection method used capillary gas chromatography and the instrument was HP6890 Series gas chromatograph. Fatty acid standards and related reagents are German company products. The determination of serum fatty acids was done mainly at the Institute of Clinical Chemistry at Ulm University in Germany. The results of test and analysis show that the main fatty acids in healthy adults are determined by their levels of free fatty acids: C18:1, C16:0, C18:2, C18:0, C20:4, C20:0, C22:6. , C14:0; total fatty acids can be ranked as follows: C18:2, C16:0, C18:1, C18:0, C20:4, C22 6, C20:0, C14:0. The ratio of free fatty acid to total fatty acid content is about 1:7; the ratio of saturated fatty acid to unsaturated fatty acid in free fatty acid is 1:1.5, and in total fatty acid is 1:1.9, indicating total fatty acid Unsaturated fatty acid content increased significantly. The content of total serum fatty acids in female healthy adults was significantly higher than that in males, except for C20:0 and C20:4, there were significant differences (P<0.05). However, there was no significant difference in free fatty acid between male and female healthy adults (P>0.05). Of the 8 free fatty acids in healthy adults, oleic acid (C18:1) increased with age, with the highest in the 50-59 age group and significant compared with other age groups (P<0.05).