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目的建立一种简便、快速、准确检测人心肌肌钙蛋白 T(c Tn T)的胶体金免疫层析法 (GICA)。方法制备的胶体金标记抗 c Tn T单克隆抗体 3F7,生物素标记另一株抗体 2 H8,链霉亲和素结合于硝酸纤维素膜上 ,制成免疫层析试纸条。血清中 c Tn T与测试条两种抗体结合后 ,沿硝酸纤维素膜移动 ,与链霉亲和素交联形成肉眼可见的红色线条。结果测试条灵敏度可达 0 .5 ng/ml。检测 30例急性心肌梗塞 (AMI)患者血清 c Tn T水平 ,并与国外同类产品比较 ,符合率达 92 %。结论本方法特异性强、灵敏度高、简便快速 ,可广泛应用于急性心肌梗塞的早期诊断
Objective To establish a simple, rapid and accurate colloidal gold immunochromatographic assay (GICA) for the detection of human cardiac troponin T (cTn T). The colloidal gold labeled anti-c Tn T monoclonal antibody 3F7 prepared by the method and the other antibody labeled with biotin 2 H8 were bound to the nitrocellulose membrane to prepare an immunochromatographic test strip. Serum cTn T and the test strip after two antibodies, along the nitrocellulose membrane movement, and streptavidin cross-linked to form a visible red lines. Results The sensitivity of the test strip was 0.5 ng / ml. Serum levels of cTn T were detected in 30 patients with acute myocardial infarction (AMI) and compared with similar foreign products, the coincidence rate reached 92%. Conclusion This method is specific, sensitive, simple and rapid and can be widely used in the early diagnosis of acute myocardial infarction