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目的:探讨子宫颈上皮正常对照组织、非典型增生组织及子宫颈癌组织中树突状细胞(DC)的形态分布改变及肿瘤坏死因子-α(TNF-α)、c-myc表达的意义,以及它们与肿瘤临床病理参数间的关系。方法:应用免疫组化S-P法检测子宫颈上皮正常对照组织、非典型增生组织各15例及40例子宫颈癌组中DC的形态分布特征及TNF-αc、-myc的表达。结果:子宫颈上皮非典型增生组及子宫颈癌组中DC数量显著高于正常对照组(P<0.001);子宫颈上皮非典型增生组中DC呈树枝状,有较多分支,子宫颈癌组中DC散在分布,位于癌巢中的DC多呈椭圆形或圆形,而位于间质中的DC呈树枝状,有较多分支;TNF-αc、-myc的表达在子宫颈癌组、非典型增生组及正常对照组间差异具有统计学意义(P<0.001)。子宫颈癌中DC的表达与患者的年龄、病理分级、临床分期及肿瘤直径有关(P<0.05);TNF-α的表达与患者的临床分期及肿瘤直径有关,而c-myc的表达仅与患者的临床分期有关(P<0.05)。结论:DC、TNF-α、c-myc在子宫颈上皮癌变过程及发展中起重要作用,并且与肿瘤临床病理有密切关系。
Objective: To investigate the morphological changes of dendritic cells (DCs) and the expression of tumor necrosis factor-α (TNF-α) and c-myc in normal cervical epithelial tissue, atypical hyperplasia and cervical cancer, And their relationship with clinicopathological parameters of the tumor. Methods: Immunohistochemical S-P method was used to detect the morphological distribution of DCs and the expression of TNF-αc and -myc in 15 cases of cervical epithelial normal control tissues and atypical hyperplastic tissues and 40 cases of cervical cancer cases. Results: The number of DC in atypical hyperplasia group and cervical cancer group was significantly higher than that in the normal control group (P <0.001). DC in the cervical epithelial atypical hyperplasia group was dendritic with more branches and cervical cancer DC in the group scattered, most of the DC located in the cancer nest oval or round, while in the interstitial DC was dendritic, more branches; TNF-αc, -myc expression in cervical cancer group, There was significant difference between atypical hyperplasia group and normal control group (P <0.001). The expression of DC in cervical cancer was related to the age, pathological grade, clinical stage and tumor diameter (P <0.05). The expression of TNF-α was related to the clinical stage and tumor diameter, while the expression of c-myc was only The patient’s clinical stage (P <0.05). Conclusion: DC, TNF-α and c-myc play an important role in the carcinogenesis and progression of cervical epithelial carcinogenesis and are closely related to the clinicopathological features of the tumor.