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目的研究抗心肌肌凝蛋白重链自身抗体(AMHCA)IgG亚类的产生与腹主动脉缩窄(AAC)、冠状动脉结扎(LCL)术后大鼠慢性心力衰竭(CHF)之间的关系。方法应用AAC术与LCL术建立两种CHF大鼠模型,以合成的大鼠心肌肌凝蛋白重链部分肽段(1135-1150位氨基酸残基)为合成肽抗原,采用间接ELISA方法检测大鼠血清中AMHCA IgG亚类的水平及动态变化。结果两组模型大鼠手术处理后1～2周AMHCA IgG1、IgG2a和IgG2b亚类阳性率较手术完成时均增高(P<0.05)。两组大鼠模型术后1～2周IgG1、IgG2a和IgG2b自身抗体阳性率和抗体滴度开始增高,3～4周达峰值,5～6周后缓慢下降。结论AAC和LCL组大鼠模型在CHF的发生、发展过程中均有AMHCA IgG亚类的产生,并呈产生、维持、衰减的动态变化。
Objective To investigate the relationship between the occurrence of IgG subclass against AMHCA and abdominal aortic constriction (AAC) and postoperative coronary artery ligation (LCL) in rats with chronic heart failure (CHF). Methods Two kinds of rat model of CHF were established by AAC and LCL. The synthetic myosin heavy chain partial peptide (amino acid residues 1135-1150) was used as the synthetic peptide antigen. The indirect ELISA was used to detect the rat Serum AMHCA IgG subclass levels and dynamic changes. Results The positive rates of AMHCA IgG1, IgG2a and IgG2b subclasses in the two groups of rats after operation were significantly higher than those at the completion of surgery (P <0.05). The positive rates of IgG1, IgG2a and IgG2b autoantibodies and antibody titers in the two groups of rats increased from 1 to 2 weeks after operation, reaching the peak at 3 to 4 weeks and slowly decreasing from 5 to 6 weeks after operation. Conclusions The AMHCA IgG subclass is produced in both the AAC and LCL rat models during the development and progression of CHF, with a dynamic change of production, maintenance and attenuation.