目的　建立一种虹膜色素上皮 (iris pigmentepithelium,IPE)细胞培养方法。　方法　使用酶消化加机械分离的方法培养 IPE细胞并进行组织学观察。　结果　用该方法可成功培养 IPE细胞。培养的 IPE细胞在原代及传代生长过程中同视网膜色素上皮 (retinal pigmentepithelium,RPE)细胞没有明显区别。　结论　酶消化加机械分离的方法是培养 IPE的理想方法 Objective To establish an iris pigmentepithelium (IPE) cell culture method. Methods IPE cells were cultured by enzymatic digestion and mechanical separation and observed histologically. Results IPE cells can be successfully cultured by this method. The cultured IPE cells did not differ significantly from the retinal pigmentepithelium (RPE) cells during primary and passage growth. Conclusions Enzymatic digestion plus mechanical separation is the ideal method for culturing IPE