论文部分内容阅读
目的研究咖啡酸苯乙酯(CAPE)对糖尿病大鼠肾的保护作用及其机制。方法用高脂高糖喂养加链脲佐菌素(STZ)损伤建立糖尿病大鼠模型。将造模大鼠按照体重随机分为模型组和实验组,同时设立正常组。实验组,每日腹腔注射CAPE 10μmol·kg~(-1),其余2组腹腔注射生理盐水,连续4周后,检测血糖(BG)、总胆固醇(TC)、三酰甘油(TG)及肾组织中丙二醛(MDA)、蛋白羰基化(PCO)、超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性;对肾组织做常规HE染色并显微镜下观察组织变化。结果与模型组相比,实验组大鼠血糖和血脂水平明显降低(P<0.01),肾中的SOD、CAT活性明显升高(P<0.01),肾组织MDA、PCO含量显著降低(P<0.01)。结论咖啡酸苯乙酯对糖尿病大鼠肾具有一定的保护作用,其机制可能与其抗氧化作用有关。
Objective To study the protective effect of caffeic acid phenethyl ester (CAPE) on the kidney of diabetic rats and its mechanism. Methods Diabetic rats were induced by high-fat and high-sucrose feeding and streptozotocin (STZ) injury. The model rats were randomly divided into model group and experimental group according to body weight, meanwhile normal group was established. In the experimental group, intraperitoneal injection of 10 μmol · kg -1 CAPE was given intraperitoneally, and the other two groups were given intraperitoneal injection of normal saline for four weeks. Blood glucose (BG), total cholesterol (TC), triglyceride (TG) The activity of malondialdehyde (MDA), protein carbonylation (PCO), superoxide dismutase (SOD) and catalase (CAT) in the tissue were measured. The histological changes of the kidney were observed by conventional HE staining and microscopy. Results Compared with the model group, the levels of blood glucose and blood lipids in the experimental group were significantly decreased (P <0.01), the activities of SOD and CAT in the kidney were significantly increased (P <0.01), and the contents of MDA and PCO in the experimental group were significantly decreased (P < 0.01). Conclusion Caffeic acid phenethyl ester has a protective effect on the kidney of diabetic rats, and its mechanism may be related to its antioxidative effect.