In hospital isolation rooms, door operation can lead to containment failures and airbe pathogen dispersal into the surrounding spaces. Sliding doors can reduce the containment failure arising from the door motion induced airflows, as compared to the hinged doors that are typically used in healthcare facilities. Such airflow leakage can be measured quantitatively using tracer gas techniques, but detailed observation of the turbulent flow features is very difficult. However, a comprehensive understanding of these flows is important when designing doors to further reduce such containment failures. Experiments and Computational Fluid Dynamics (CFD) modelling, by using Large-Eddy Simulation (LES) flow solver, were used to study airflow patts in a full-scale mock-up, consisting of a sliding door separating two identical rooms (i.e. one isolation room attached to an antechamber). A single sliding door open/ hold-open/ closing cycle was studied. Additional variables included human passage through the doorway and imposing a temperature difference between the two rooms. The general structures of computationally-simulated flow features were validated by comparing the results to smoke visualizations of identical full-scale experimental set-ups. It was found that without passage the air volume leakage across the doorway was first dominated by vortex shedding in the wake of the door, but during a prolonged hold-open period a possible temperature difference soon became the predominant driving force. Passage generates a short and powerful pulse of leakage flow rate even if the walker stops to wait for the door to open.