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作者评价了在造血生长因子作用下,通过提高AML原始细胞增殖活性,可能增强Ara-C对细胞的杀伤效果。取10例未治AML患者的外周血,用Ficoll-Hypaque密度梯度离心法分离出白血病细胞,浓度配成1×10~6/ml。以细胞系5637培养的上清液作为集落刺激活性的来源,以液体培养基IMDM+10%胎牛血清(FCS)培养3天。加入或不加入生长因子,后者作对照组;前者加人体重组生长因子ILI(β,5U/ml)或IL-3(3U/ml)为实验组。在连续培养3天的最后24小时均加入Ara-C(3μg/ml/12小时)。两者作用后,残存白血病干细胞数量,可通过半固体培养基克隆分析技术(~3H TdR摄取率)进行测定,结果表明
The authors assessed that under the effect of hematopoietic growth factors, Ara-C may enhance the killing effect of cells by increasing the proliferative activity of AML protocells. The peripheral blood of 10 untreated AML patients was taken and the leukemic cells were isolated by Ficoll-Hypaque density gradient centrifugation at a concentration of 1×10 6 /ml. Supernatant cultured in the cell line 5637 was used as a source of colony-stimulating activity and cultured for 3 days in a liquid medium IMDM+10% fetal bovine serum (FCS). With or without addition of growth factors, the latter served as a control group; the former was supplemented with human recombinant growth factor ILI (β, 5 U/ml) or IL-3 (3 U/ml) as the experimental group. Ara-C (3 μg/ml/12 hours) was added during the last 24 hours of continuous culture for 3 days. After the two effects, the number of remaining leukemic stem cells can be determined by semi-solid medium cloning analysis technique (~3H TdR uptake rate).