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目的以赤芝为基础建立灵芝三萜类化合物高效薄层荧光色谱指纹图谱,并与赤芝的近缘种无柄灵芝、平盖灵芝等的指纹图谱进行比较分析。方法硅胶GF254高效预制薄层板,以正己烷-乙酸乙酯-甲醇-甲酸(30∶30∶2∶0.2)为展开剂展开,展至约5cm,再以正己烷-乙酸乙酯-甲醇-甲酸(30∶30∶1∶0.2)为展开剂进行二次展开,10%硫酸乙醇溶液显色后获得薄层荧光色谱指纹图谱,经指纹图谱系统解决方案软件(ChromafingerTM)生成共有模式,并进行相似度和主成分分析。结果赤芝薄层荧光色谱指纹图谱由9个特征荧光条斑组成。荧光色谱图像与轮廓扫描图谱结合分析比较,建立了赤芝指纹图谱模式。赤芝与紫芝、无柄灵芝、平盖灵芝、薄树芝、黑芝等在三萜类化合物方面区别较大。结论通过指纹图谱考察,当前市场灵芝类型主要是赤芝、紫芝为主流,但两者区别较大,其他灵芝的近缘种均为代用品,不可做灵芝使用,应加以区别。
Objective To establish a high-performance TLC fingerprint chromatogram of Ganoderma lucidum triterpenes based on Ganoderma lucidum, and to compare with the fingerprints of Ganoderma lucidum and Ganoderma lucidum. Methods Silica gel GF254 prefabricated plate was developed with n-hexane-ethyl acetate-methanol-formic acid (30:30:2:0.2) Formic acid (30: 30: 1: 0.2) was used as the developing solvent for the second expansion. The TLC chromatogram was obtained after the color was developed with 10% sulfuric acid in ethanol and the common mode was generated by the ChromafingerTM Similarity and principal component analysis. Results Chlamys farreri TLC fingerprinting consisted of nine characteristic fluorescent streaks. Fluorescence chromatograms and contour scan spectra were analyzed and compared to establish the Chi Zhi fingerprint pattern. Ganoderma lucidum and Ganoderma lucidum, Ganoderma lucidum, flat Ganoderma lucidum, thin chuanzhi, black sesame and other compounds in the triterpene distinction. Conclusion According to the study of fingerprints, Ganoderma lucidum is mainly Ganoderma lucidum and Ganoderma lucidum in the current market, but the difference between the two is greater. Other Ganoderma lucidum related species are substitutes, and Ganoderma lucidum should not be used, and should be distinguished.