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目的:建立菊花药材指纹图谱检测方法,并对硫磺熏蒸前后菊花药材的指纹图谱进行分析比较。方法:采用高效液相色谱法,色谱柱为Waters C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%磷酸水溶液,梯度洗脱;柱温35℃,检测波长268 nm。结果:测定未经硫磺熏蒸和经过硫磺熏蒸的菊花药材样品共11批,建立了菊花药材HPLC指纹图谱的共有模式,确立12个共有峰,并且采用聚类分析方法对11批样品进行分析比较。结论:该方法准确可靠,重复性好,可用于硫磺熏蒸前后菊花药材的质量评价与控制。
OBJECTIVE: To establish a method for fingerprint determination of medicinal materials of chrysanthemum and to compare and analyze the fingerprints of medicinal materials of chrysanthemum before and after sulfur fumigation. Methods: The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with gradient C18 (250 mm × 4.6 mm, 5 μm) by using high performance liquid chromatography. The column temperature was 35 ℃ and the detection wavelength was 268 nm. Results: A total of 11 batches of chrysanthemum medicinal samples without sulfur fumigation and sulfur fumigation were determined. The common pattern of HPLC fingerprints of medicinal plants was established, 12 common peaks were established, and 11 batches of samples were analyzed and compared by cluster analysis. Conclusion: The method is accurate and reliable with good repeatability and can be used for quality evaluation and control of chrysanthemum before and after sulfur fumigation.