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目的 研究大肠癌中ＣＤ４４ Ｖ６ 蛋白的表达及其对大肠癌进展和转移的影响。方法 收集大肠癌新鲜组织标本５２ 例（ 其中包括无转移病例的原发灶２９ 例及有转移的淋巴结２３ 例） 、腺瘤性息肉１６ 例及正常大肠粘膜组织１１ 例。应用ＬＳＡＢ 免疫组织化学染色技术检测上述各种组织中ＣＤ４４ Ｖ６ 的表达。结果 无转移大肠癌癌灶中的ＣＤ４４ Ｖ６ 的阳性表达率为５１ ．７ ％ （１５／２９） ，转移灶中的ＣＤ４４ Ｖ６ 表达率为８７ ．０ ％ （２０／２３） ，腺瘤性息肉为１８ ．８ ％ （３／１６） ，而正常大肠粘膜组织为无阳性表达（０／１１） 。大肠癌组织中ＣＤ４４ Ｖ６ 的阳性表达率较腺瘤性息肉（ Ｐ＜ ０ ．０１） 和正常大肠粘膜组织（ Ｐ ＜ ０ ．０１） 为高，淋巴结转移癌ＣＤ４４ Ｖ６ 阳性表达率较无转移的原发癌明显为高（ Ｐ＜ ０ ．０１） ，同时发现大肠癌中ＣＤ４４ Ｖ６ 的阳性表达率与肿瘤分化程度、大体类型、大小和部位无关。结论 对大肠癌组织进行ＣＤ４４Ｖ６ 蛋白的检测，有助于预测其进展程度和对淋巴结转移的诊断。
Objective To study the expression of CD44 V6 protein in colorectal carcinoma and its effect on the progression and metastasis of colorectal carcinoma. Methods Colorectal cancer fresh tissue samples were collected in 52 cases (including 29 cases of primary tumor without metastasis and 23 cases of lymph node metastasis), 16 cases of adenomatous polyps and 11 cases of normal colorectal mucosa. LSAB immunohistochemical staining technique was used to detect the expression of CD44 V6 in various tissues above. Results The positive rate of CD44 V6 in non-metastatic colorectal cancer was 51. 7% (15/29), and the expression rate of CD44 V6 in metastasis was 87. 0% (20/23), adenomatous polyps 18. 8% (3/16), while no expression was found in normal colorectal mucosa (0/11). The positive rate of CD44 V6 in colorectal cancer was higher than that in adenomatous polyps (P <0.01) and normal colorectal mucosa (P <0.01), and the positive rate of CD44 V6 in lymph node metastasis was higher than that in non-metastatic Significantly higher incidence of cancer (P <0.01), and found that the positive expression of CD44 V6 in colorectal cancer and tumor differentiation, general type, size and location has nothing to do. Conclusion The detection of CD44V6 protein in colorectal cancer will be helpful for predicting its progression and diagnosis of lymph node metastasis.