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目的探讨头孢曲松钠羊膜腔内和静脉给药对大肠埃希菌所致宫内感染的晚孕SD大鼠胎盘组织白细胞介素-6(IL-6)及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响。方法妊娠15d的SD孕鼠36只,完全随机分成4组,每组9只。①妊娠第15天,所有孕鼠一侧宫角行1010/L大肠埃希菌菌液0.2ml接种。②妊娠第16天,羊膜腔内给药组(IUA)和对照组(IUC)分别于接种侧各羊膜腔内注射头孢曲松钠和等体积的生理盐水,静脉给药组(IVA)和对照组(IVC)母鼠尾静脉分别注射头孢曲松钠和等体积的生理盐水。③4组孕鼠自妊娠第17天起,每组每天随机处死3只,随机抽取其接种侧宫角顶端3个胎囊之一的胎盘行IL-6、MMP-9免疫组化染色后行半定量分析。结果胎盘IL-6和MMP-9表达水平在给药后第1、2、3天,各给药组低于各相应对照组(P<0.05),IUA组低于IVA组(P<0.05),IUC组高于IVC组(P<0.05)。IUA组和IVA组IL-6水平于给药后第2天最低(P<0.05),IUC组和IVC组IL-6表达水平及各组MMP-9表达水平逐日增高(P<0.05)。结论单次头孢曲松钠给药治疗晚孕SD大鼠大肠埃希菌宫内感染,羊膜腔内给药较母鼠静脉途径更有效,其可通过抑制IL-6和MMP-9在胎盘组织中的表达水平,降低炎症反应水平和胎膜早破的风险。
Objective To investigate the effects of ceftriaxone sodium on the placental tissue interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) in neonatal SD rats with intrauterine infection caused by Escherichia coli -9, MMP-9) expression. Methods Thirty-six pregnant SD rats were randomly divided into 4 groups (n = 9). ① on the 15th day of gestation, all the pregnant rats on the side of the cornwall 1010 / L Escherichia coli solution 0.2ml inoculation. ② On the 16th day of gestation, intra-amniotic cavity administration group (IUA) and control group (IUC) were injected with ceftriaxone sodium and the same volume of normal saline, intravenous administration group (IVA) and control Group IVC female mice tail vein injection of ceftriaxone sodium and an equal volume of saline. (3) Pregnant mice in group 4 were randomly sacrificed at day 17 of gestation, and randomly selected three placentas of one of the three fetal sacs at the top of the inoculation side to perform IL-6 and MMP-9 immunohistochemistry. Quantitative analysis. Results The levels of IL-6 and MMP-9 in the placenta were lower than those in the IVA group (P <0.05) , IUC group was higher than IVC group (P <0.05). The level of IL-6 in IUA group and IVA group was the lowest on the 2nd day after administration (P <0.05). The IL-6 expression level in IUC group and IVC group increased day by day (P <0.05). Conclusion Single ceftriaxone sodium administration in the treatment of intrauterine infection of Escherichia coli in late pregnancy SD rats is more effective than intravenous administration of amniotic fluid in female rats. It can inhibit IL-6 and MMP-9 expression in placenta In the expression level, reduce the level of inflammation and the risk of premature rupture of membranes.