论文部分内容阅读
目的采用血管紧张素Ⅱ(angiotensin Ⅱ,AngⅡ)作为诱导剂,体外诱导成人脂肪间充质干细胞(adipose-de-rived mesenchymal stem cells,ADMSCs)向心肌细胞方向分化。方法自成人脂肪组织中分离培养ADMSCs,分别用0.05、0.10和0.20μmol/L3种不同浓度的AngⅡ诱导ADMSCs,同时设立对照组,倒置相差显微镜及透射电子显微镜下观察细胞的形态学特征,免疫细胞化学染色方法检测心肌特异性肌钙蛋白-I(cardiac troponin-I,cTn-I),计算转化率。结果诱导后第3周时,0.20μmol/L组可见cTn-I阳性细胞表达,第4周时0.10μmol/L组和0.20μmol/L组均有cTn-I阳性细胞表达,但0.20μmol/L组的阳性细胞数明显多于前组,且细胞形态变化显著。结论AngⅡ在体外可以诱导ADMSCs分化为心肌样细胞,其诱导分化的最佳浓度为0.20μmol/L。
Objective To induce the differentiation of adult adipose-derived mesenchymal stem cells (ADMSCs) into cardiomyocytes in vitro using angiotensin Ⅱ (AngⅡ) as an inducer. Methods ADMSCs were isolated and cultured from adult adipose tissue. ADMSCs were induced by different concentrations of AngⅡ (0.05, 0.10 and 0.20 μmol / L) respectively. At the same time, ADMSCs were cultured in the control group. The morphological features of the cells were observed under inverted phase contrast microscope and transmission electron microscope. The cardiac-specific troponin-I (cTn-I) was detected by chemical staining and the conversion rate was calculated. Results At the third week after induction, cTn-I positive cells were observed in the 0.20μmol / L group and cTn-I positive cells in the 0.10μmol / L group and 0.20μmol / L group at the 4th week, The number of positive cells in the group was significantly more than that in the former group, and the cell morphology changed significantly. Conclusion AngⅡ can induce ADMSCs to differentiate into cardiomyocytes in vitro, and the optimal concentration of ADAMs for inducing differentiation is 0.20μmol / L.