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目的了解我国恶性疟原虫云南地理株核糖体小亚基rDNA的核苷酸序列。方法根据已知恶性疟原虫核糖体小亚基rDNA序列．在其基因的中下游分别设计引物，采用聚合酶链式反应（PCR）技术，从云南省来源的恶性疟体外培养原虫的基因组DNA中扩增出的基因片段酶切后将其插入pUC118／119载体，用Biosystems373ADNA序列分析仪测定。结果我国恶性疟原虫云南地理株核糖体小亚基rDNA核苷酸序列，与巴西IMTM22／7G8株的序列比较，在该基困的第1563位有一个A被T替换。结论该突变点位于真核生物核糖作小亚基rRNA基因已知序列种类的第三个可变区域内。
Objective To understand the nucleotide sequence of ribosomal small subunit rDNA of P. falciparum Yunnan geographic strain in China. Methods Based on the known rDNA sequences of the small subunit of Plasmodium falciparum. Primers were designed in the middle and lower reaches of the gene respectively. The gene fragment amplified from genomic DNA of P. falciparum cultured in Yunnan Province was digested by PCR and inserted into pUC118 / 119 The vector was measured using a Biosystems 373ADNA sequencer. Results The rDNA nucleotide sequence of ribosomal small subunit of P. falciparum Yunnan geographic strain was compared with the sequence of IMTM22 / 7G8 strain in Brazil. Conclusion This mutation is located in the third variable region of the known sequence of rRNA gene of eukaryotic ribosomal small subunit.