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目的 评价蛋白涂层镍钛合金支架向血管局部转基因的可行性和效率。材料与方法 镍钛合金支架由明胶蛋白涂层 ,浸入含编码外源性P2 1WAF1/CIP1基因的复制缺陷型腺病毒溶液中 5min ,然后通过 7F长鞘将支架送入犬髂动脉狭窄模型中的一侧髂总动脉 (n =4 ) ,将未浸泡过基因溶液的支架送入另一侧髂总动脉 (n =4 )。在支架置入后 7天处死动物 ,RT PCR和免疫组化染色评价基因转移效率。结果 所有转基因血管经RT PCR扩增出P2 1特异条带 ,免疫组化染色显示在转基因血管内膜、中膜及外膜均有外源性基因表达 ,对照组血管未显示阳性表达。结论 蛋白涂层镍钛合金支架向血管内转基因是可行及有效的 ,可能成为血管成形术后再狭窄基因治疗的有效转基因工具
Objective To evaluate the feasibility and efficiency of protein-coated nickel-titanium stent to local vascular transgene. Materials and Methods Nitinol stents were coated with gelatin protein and immersed in a replication deficient adenovirus solution containing exogenous P2 1 WAF1 / CIP1 gene for 5 min, then introduced into a canine iliac artery stenosis model by a 7F long sheath One side of the common iliac artery (n = 4), the stent was not soaked in the gene solution into the other side of the common iliac artery (n = 4). Animals were sacrificed 7 days after stent placement and gene transfer efficiency was assessed by RT PCR and immunohistochemistry. Results All transgenic blood vessels were amplified by RT PCR. The results of immunohistochemical staining showed that exogenous gene was expressed in the intima, media and adventitia of the transgenic vessels, but not in the control group. Conclusions The protein-coated nickel-titanium stent is feasible and effective for intravascular transplatation and may be an effective transgenic tool for gene therapy of restenosis after angioplasty