论文部分内容阅读
目的对河南省林州地区食管癌高发区食管鳞癌患者(食管癌组)和健康人血清(对照组)蛋白质组进行比较研究,确立食管癌的蛋白质指纹诊断模型,并筛选与食管癌淋巴结转移相关的蛋白质。方法采用IMAC3芯片及表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)检测61例食管癌患者和50例健康人血清,用Bio-MarkerWizard软件对芯片检测得到的蛋白质相对含量进行处理及方差分析。并对25例发生淋巴结转移的食管癌患者(转移组),36例无淋巴结转移患者(未转移组)与对照组进行了对比分析。结果以相对分子质量(Mr)为9439.58、6627.21、2867.65、4494.08、7762.68、6835.32、4095.947种蛋白质组建立的决策树模型,对食管癌测试的准确率、敏感度和特异度分别为85.6%、88.5%和82.0%。转移组、未转移组与对照组3组相比,有15种蛋白差异有统计学意义。其中转移组和未转移组相比有2种蛋白差异有统计学意义(Mr分别为11742.48、9294.44)。结论以上述7种蛋白质建立的诊断模型用于食管癌的诊断,具有较高灵敏度和特异度;筛选出2种与食管癌转移密切相关蛋白质,后者为进一步建立食管癌转移相关肿瘤标志物提供了重要线索。
Objective To compare the proteome of patients with esophageal squamous cell carcinoma (ESCC) and healthy human serum (control group) with high incidence of esophageal cancer in Linzhou area of Henan Province and to establish a protein fingerprinting diagnostic model of esophageal cancer and to screen the proteome of esophageal cancer with lymph node metastasis Related proteins. Methods Serum samples of 61 patients with esophageal cancer and 50 healthy individuals were detected by IMAC3 chip and surface enhanced laser desorption / ionization time of flight mass spectrometry (SELDI-TOF-MS). Bio-MarkerWizard software was used to process the relative protein content detected by the chip. variance analysis. Twenty-five patients with esophageal cancer who had lymph node metastasis (metastasis group) and 36 patients without lymph node metastasis (without metastasis group) were compared with the control group. Results The decision tree model with relative molecular mass (Mr) of 9439.58, 6627.21, 2867.65, 4494.08, 7762.68, 6835.32 and 4095.947 proteomes was 85.6%, 88.5 % And 82.0%. There were 15 proteins in the metastasis group, non-metastasis group and control group. The difference between the two groups in metastasis group and non-metastasis group was statistically significant (Mr 11742.48,9294.44 respectively). Conclusion The diagnostic models established by the above seven kinds of proteins are of high sensitivity and specificity for the diagnosis of esophageal cancer. Two kinds of proteins that are closely related to the metastasis of esophageal cancer were screened, and the latter were selected to further establish the metastatic tumor markers of esophageal cancer The important clues.