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目的:建立测定刺齿凤尾蕨中抗肿瘤成分5F含量的方法,并测定其不同部位中5F的含量。方法:采用高效液相色谱法。色谱柱为HypersilC1(8250mm×4.6mm,5μm),流动相为甲醇-1%醋酸水溶液(梯度洗脱),流速为1.0mL·min-1,检测波长为242nm,柱温为30℃。结果:5F检测浓度在6～96μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9981);低、中、高浓度对照品加入量对应的平均加样回收率分别为103.33%、100.84%、100.39%,RSD分别为5.86%、3.05%、1.02%(n均为6)。5F在刺齿凤尾蕨叶中含量最高,茎中含量其次,根中含量最低。结论:本方法稳定、快速且杂质干扰小,可用于刺齿凤尾蕨中5F的含量测定。
OBJECTIVE: To establish a method for determination of anti-tumor 5F content in Pteris fern, and to determine the content of 5F in different parts of it. Methods: Using high performance liquid chromatography. The chromatographic column was HypersilC1 (8250mm × 4.6mm, 5μm). The mobile phase was methanol-1% acetic acid aqueous solution (gradient elution). The flow rate was 1.0mL · min-1. The detection wavelength was 242nm and the column temperature was 30 ℃. Results: The calibration curve of 5F showed a good linear relationship with the peak area (r = 0.9981) in the range of 6 ~ 96μg · mL-1. The average recoveries of low, medium and high concentrations of reference substance were 103.33 %, 100.84% and 100.39%, respectively. The RSDs were 5.86%, 3.05% and 1.02% respectively (n = 6). 5F was the highest content in the fern leaves, followed by the content in the stems and lowest in the roots. Conclusion: The method is stable, rapid and less interference of impurities. It can be used to determine the content of 5F in Pteris fern.