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植物名称:梨矮化砧木PDR54、PDR4(Pyrusussuriensis×P.communis)。材料类别:茎尖和带腋芽的茎段。培养条件:诱导芽分化培养基:1/2 MS(有机、铁盐为全量)+BAlmg/L(单位下同)+NAA0.1;继代增殖培养基:(1)1/2MS+BAl+NAA0.2+GA0.05;(2)MS+BAl+IBA0.2～0.5+GA0.1;益根培养基:(1)1/2MS十NAA0.5～1;(2)1/2MS+IBA0.25～2;(3)1/2MS+IAA1.5～2。诱导芽
Plant Name: Pear Dwarf Rootstock PDR54, PDR4 (Pyrusussuriensis × P.communis). Material category: shoot tips and stem segments with axillary buds. Culture conditions: Induction sprout differentiation medium: 1/2 MS (total amount of organic and iron salts) + BAlmg / L (same below) + NAA0.1; subculture medium: (1) 1/2 MS + BAl + NAA0.2 + GA0.05; (2) MS + BAl + IBA0.2 ~ 0.5 + GA0.1; Eagan culture medium: (1) 1/2 MS ten NAA 0.5 ~ 1; IBA0.25 ~ 2; (3) 1 / 2MS + IAA1.5 ~ 2. Induce buds