在色谱分离过程中强组分为零的情况极少出现，根据统计热力学研究得出的保留值分式ｌｎｋ＝ａ＋ｂｌｎＣＢ，当ＣＢ趋于零时，其色谱保留值ｋ应趋于无穷；本文在进行菊酯类农药的手性分离过程中有幸遇到ＣＢ值极低直至等于零的色谱条件，此时，其色谱保留值ｋ逐步趋于一个定值，而并非趋于无穷，此试验结果为色谱保留值公式的理论修正提供了实验依据． In the chromatographic separation process is rare in the case of zero component, according to the statistical thermodynamic study of retention value fraction lnk = a + blnCB, when CB tends to zero, the chromatographic retention k should tend to infinity; In this paper, chrysanthemum pesticides in the chiral separation process fortunate enough to encounter the CB value is extremely low until zero chromatographic conditions, then, the chromatographic retention value k gradually toward a fixed value, not to infinity, this test The results provide experimental evidence for the theoretical modification of the formula of chromatographic retention.