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目的研究叠鞘石斛水提取物(DDWEs)对小肠结肠炎耶尔森菌的抗菌活性及抗菌机制。方法采用96孔板微量肉汤稀释法以及平板培养法测定DDWEs对小肠结肠炎耶尔森菌的最小抑菌浓度(MIC)和最低杀菌浓度(MBC);并通过测定培养液的电导率和大分子物质吸光度(OD)值以及DAPI荧光染色后的荧光观测,研究DDWEs对小肠结肠炎耶尔森菌细胞膜和核酸合成的影响。结果 DDWEs对小肠结肠炎耶尔森菌MIC和MBC浓度均为100 mg/ml;DDWEs作用前后,小肠结肠炎耶尔森菌培养液的电导率增加,但大分子物质的OD值变化不大,而DAPI染色后的荧光强度则产生显著变化。结论 DDWEs对小肠结肠炎耶尔森菌具有较强的抗菌作用,其抗菌机制可能涉及影响小肠结肠炎耶尔森菌的细胞膜通透性和核酸合成。
Objective To study the antibacterial activity and antibacterial activity of DDWEs against Yersinia enterocolitica. Methods The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of DDWEs against Yersinia enterocolitica were determined by 96-well microtiter plate dilution method and plate culture method. By measuring the conductivity and large Molecular material absorbance (OD) value and fluorescence observation after DAPI fluorescence staining to study the effects of DDWEs on Y. enterocolitica membrane and nucleic acid synthesis. Results The concentration of MIC and MBC of Yersinia enterocolitica were both 100 mg / ml for DDWEs. Before and after DDWEs treatment, the conductivity of Yersinia enterocolitica culture medium increased, but the OD values of macromolecules did not change much, However, the fluorescence intensity after DAPI staining changed significantly. Conclusion DDWEs have strong antibacterial activity against Yersinia enterocolitica, and its antibacterial mechanism may involve the cell membrane permeability and nucleic acid synthesis of Yersinia enterocolitica.