论文部分内容阅读
In the present study, a perfusion system of dispersed cells was used to investigate theeffects of LHRH pulse amplitude and frequency, and LHRH continuous stimulation on LHsecretion by anterior pituitary cells of adult male rats. The results have shown that, in therange of LHRH concentrations from 1×10~(-10) to 1 ×10~(-6) mol/L, the dose-response curve of LHsecretion was linear. LHRH pulse frequency generated a biphasic LH response: increasingLHRH pulse frequency increased the basal LH secretion and decreased LH/pulse. When1 ×10~(-9), mol/L or greater LHRH was given at frequencies of 3 pulses/h or higher, it wasobserved that a maximal LH peak was induced and then the LH release declined progres-sively to its LH basal level, i.e. LHRH self-priming effect and LH desensitization occurred.Enhancement of amplitude of LHRH pulses could reduce pulse frequency required for prim-ing. Increases in frequency of LHRH pulses with high amplitude would provoke the prim-ing effect more quickly. In addition, continuou
In the present study, a perfusion system of dispersed cells was used to investigate the effects of LHRH pulse amplitude and frequency, and LHRH continuous stimulation on LH secretion by anterior pituitary cells of adult male rats. The results have shown that in therange of LHRH concentrations from The dose-response curve of LHsecretion was linear. LHRH pulse frequency generated biphasic LH response: increasing LHRH pulse frequency increased the basal LH secretion and decreased When 1 × 10 ~ (-9), mol / L or greater LHRH was given at frequencies of 3 pulses / h or higher, it wasobserved that a maximal LH peak was induced and then the LH release declined progres-sively to its LH basal level, ie LHRH self-priming effect and LH desensitization occurred. Increment of amplitude of LHRH pulses could reduce pulse frequency required for prim-ing. Increases in frequency of LHRH pulses with high amplitude would provoke the prim-ing effect more In a ddition, continuou