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将携带有乙型肝炎病毒（ａｄｗ亚型）表面抗原（ＨＢｓＡｇ）基因和 ＨＢｓＡｇ及其前导序列（ＨＢｓＡｇ＋ｐｒｅＳ_１＋ｐｒｅＳ_２）基因的植物表达载体 ｐＲｏＫⅡ，分别导入农杆菌 ＬＢＡ４４０４中，通过叶盘转化法得到转基因番茄及其后代。 Ｓｏｕｔｈｅｒｎ ｂｌｏｔ杂交证明两种外源基因已插入到植物染色体中。ＥＬＩＳＡ检测结果表明用ＨＢｓＡｇ基因转化的番茄叶片和果实中都能表达ＨＢｓＡｇ，而用含ｐｒｅＳ的ＨＢｓＡｇ基因转化的番茄都测不出ＨＢｓＡｇ的表达。通过免疫吸附电镜观察到有表达的叶片组织可提取出典型的Ｄａｎｅ颗粒，其平均直径为 ２２ ｎｍ。
The plant expression vector pRoKII carrying HBsAg gene and HBsAg and its precursor sequence (HBsAg + preS_1 + preS_2) was introduced into Agrobacterium LBA4404, and the transgenic tomato and Its offspring Southern blot hybridization proved that two exogenous genes have been inserted into plant chromosomes. ELISA results showed that HBsAg was expressed in tomato leaves and fruits transformed with HBsAg gene, while HBsAg expression in tomato transformed with HBsAg gene containing preS was undetectable. The typical Dane particles were extracted from the expressed leaf tissue by immunoadsorption electron microscopy and the average diameter was 22 nm.