论文部分内容阅读
目的:探讨游泳运动对D-半乳糖所致衰老小鼠学习记忆和胆碱能系统功能的影响。方法:50只昆明种小鼠随机分成5组:对照组、模型组、15 min/天游泳组、30 min/天游泳组、45 min/天游泳组。除对照组外,所有小鼠连续8周皮下注射D-半乳糖复制衰老模型。运动组小鼠从第5周开始运动,连续4周。Morris水迷宫实验评价小鼠空间学习记忆能力。行为测试结束后,检测小鼠脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及皮层和海马组织中乙酰胆碱酯酶(ACh E)和胆碱乙酰化转移酶(Ch AT)的活性。结果:水迷宫实验结果显示:15 min/天和30 min/天游泳组逃避潜伏期显著短于模型组,目标象限搜索时间百分比、通过目标区域次数均显著多于模型组。酶活性检测结果显示:15min/天游泳组小鼠脑组织SOD显著高于模型组,GSH-Px活性高于模型组但无显著性,MDA含量显著低于模型组;30 min/天游泳组小鼠脑组织SOD和GSH-Px活性均显著高于模型组、MDA含量显著低于模型组;15 min/天和30 min/天游泳组皮层和海马组织中ACh E、Ch AT活性均显著高于模型组。结论:15 min/天游泳运动和30 min/天游泳运动均能提高衰老模型小鼠的空间学习记忆能力,其中以30 min/天游泳运动的效果最好,其机制可能与其提高脑组织抗氧化和清除自由基能力,增强中枢胆碱能神经系统功能有关。
Objective: To investigate the effect of swimming exercise on learning and memory and cholinergic system in aging mice induced by D-galactose. Methods: Fifty Kunming mice were randomly divided into five groups: control group, model group, 15 min / day swimming group, 30 min / day swimming group and 45 min / day swimming group. All mice except the control group were injected with D-galactose replication senescence model for 8 weeks continuously. Exercise group mice from the 5th week of exercise, continuous 4 weeks. Morris water maze test to evaluate spatial learning and memory in mice. After the behavioral test, the content of malondialdehyde (MDA) and the activities of superoxidase dismutase (SOD) and glutathione peroxidase (GSH-PX) and the activity of acetylcholinesterase in cortex and hippocampus (ACh E) and choline acetyltransferase (Ch AT) activity. Results: The results of water maze test showed that the escape latency of the swimming group at 15 min / day and 30 min / day was significantly shorter than that of the model group, the percentage of search time in the target quadrant and the number of passing the target area were significantly more than those in the model group. The results of enzyme activity test showed that SOD in brain tissue of 15min / day swimming group was significantly higher than that of model group, GSH-Px activity was higher than that of model group, while MDA content was lower than that of model group. The activity of SOD and GSH-Px in brain tissue of rats were significantly higher than that of model group, and the MDA content was significantly lower than that of model group. The activities of ACh E and Ch AT in cortex and hippocampus of 15 min / day and 30 min / Model group. CONCLUSION: Both swimming at 15 min / day and swimming at 30 min / day can improve spatial learning and memory of aging mice. Swimming at 30 min / day has the best effect, and its mechanism may be related to improving brain antioxidant capacity And the ability to scavenge free radicals and enhance the central cholinergic nervous system function.