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目的检测miRNA在肝癌中的表达谱,探讨miR-338-3p在肝癌组织中的表达及与肝癌临床病理参数的关系和意义。方法采用液相芯片技术分析20对肝癌组织与其癌旁组织中114种miRNA表达谱的差异。Real-timeRT-PCR验证另36对肝癌组织中miR-338-3p下调表达及其与肝癌临床参数的相关性。结果 31种miRNA在肝癌组织与非肝癌组织间呈现差异表达,其中12种miRNA在癌组织的表达较癌旁组织上调,19种miRNA在癌组织中表达下调。miR-338-3p下调表达程度与肝癌恶性程度、肿瘤分化程度、肝内和肝外转移及门静脉癌栓有关,与患者性别、AFP、HBsAg、是否合并肝硬变等不相关(P<0.01)。miR-338-3p的表达量肝癌组织低于非肿瘤组织,转移性肝癌低于非转移性癌组织,miR-338-3p的表达量随着肿瘤级别的增加而逐近降低(P<0.01)。结论肝癌组织与癌旁组织之间存在miRNA差异表达,其中miR-338-3p下调表达程度与肝恶性行为相关。
Objective To detect the expression of miRNA in hepatocellular carcinoma (HCC) and to investigate the relationship between the expression of miR-338-3p and the clinicopathological parameters of HCC. Methods The expression patterns of 114 miRNAs in 20 pairs of HCC tissues and adjacent tissues were analyzed by liquid-phase microarray. Real-time RT-PCR was used to verify the downregulation and expression of miR-338-3p in the other 36 pairs of hepatocellular carcinoma and its correlation with the clinical parameters of hepatocellular carcinoma. Results 31 miRNAs were differentially expressed between HCC tissues and non-HCC tissues, of which 12 miRNAs were up-regulated in cancerous tissues and 19 miRNAs were down-regulated in cancerous tissues. The down-regulated expression of miR-338-3p correlated with the degree of malignancy, the degree of tumor differentiation, intrahepatic and extrahepatic metastasis, and portal vein tumor thrombus, but not with the gender, AFP, HBsAg, cirrhosis or not (P <0.01) . The expression of miR-338-3p in HCC tissue was lower than that in non-tumor tissue and metastatic HCC tissue was lower than that in non-metastatic cancer tissue. The expression of miR-338-3p decreased with the tumor grade (P <0.01) . Conclusion There is miRNA expression difference between HCC tissues and adjacent non-cancerous tissues. The expression of miR-338-3p is related to the malignant behavior of liver.