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为充分开发利用红千层(Callistemon rigidus R.Br.)植物资源,采用水蒸汽蒸馏法提取了红千层叶的精油(CrEO),测定了CrEO的总抗氧化能力;以人工合成抗氧化剂二丁基羟基甲苯(BHT)和丁基羟基茴香醚(BHA)作为阳性对照,测定了其对羟基(OH)自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力;并采用浸液法测试了CrEO对中华按蚊四龄幼虫的毒杀效果.结果显示,CrEO的总抗氧化活性强于BHT,但弱于BHA,总抗氧化活性随浓度升高呈现显著增强的趋势.CrEO对DPPH自由基的清除率达到50%时的质量浓度(IC_(50))为6.452 mg/m L,对羟基自由基的IC50值小于1.0×10~(-5) mg/m L.在毒杀实验中,CrEO对中华按蚊四龄幼虫的24 h毒杀LC_(50)(半致死浓度)值为293.31 mg/L.本研究结果表明,CrEO具有较强的抗氧化活性,对中华按蚊幼虫具有一定的毒杀活性,具备进一步开发成为天然的抗氧化剂和植物源杀虫剂的潜力.
In order to fully exploit and utilize the plant resources of Callistemon rigidus R. Br., The essential oil (CrEO) of leaves was extracted by steam distillation, and the total antioxidant capacity of CrEO was determined. Butylhydroxytoluene (BHT) and butylated hydroxyanisole (BHA) were used as positive control. The free hydroxyl groups (OH) and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) The results showed that the total antioxidant activity of CrEO was stronger than that of BHT, but weaker than that of BHA, the total antioxidant activity increased with the increase of concentration (IC 50) of 6.452 mg / m L for the DPE free radical scavenging rate of CrEO was less than 1.0 × 10 -5 ) mg / m L. In the toxicity test, the LC50 (semi-lethal concentration) value of CrEO for the fourth instar larvae of Anopheles sinensis was 293.31 mg / L. The results of this study showed that CrEO had stronger Of the antioxidant activity of the Anopheles sinensis larvae have a certain degree of poison activity, with further development into a natural antioxidant and plant-based pesticides potential.