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目的:分析白细胞介素-23受体(Interleukin-23 receptor,IL-23R)rs17375018、rs11805303位点广西人群单核苷酸多态性。方法:对251例研究对象采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术并结合HapMap中其他地区、人种多态性数据分析IL-23R基因单核甘酸多态性。结果:rs17375018位点基因型频率为:GG型37.5%,AG型45.8%和AA型16.7%,其多态性频率分布与北京、美国犹他州、尼日利亚人群均有差异(P<0.05),与东京人群分布无差异(P>0.05)。rs1180530位点则为CC型24.7%、TC型47.8%和TT型27.5%,其多态性频率分布与美国犹他州、尼日利亚人群有差异(P<0.05),与北京及东京人群无差异(P>0.05)。结论:rs17375018位点多态性频率分布与北京、美国犹他州、尼日利亚人群及rs11805303位点多态性频率分布与美国犹他州、尼日利亚人群均有差异。
OBJECTIVE: To analyze the single nucleotide polymorphism of Guangxi population in rs17375018 and rs11805303 loci of Interleukin-23 receptor (IL-23R). Methods: A total of 251 subjects were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and other regions and polymorphisms in HapMap -23R gene single nucleotide polymorphism. Results: The genotype frequency of rs17375018 was 37.5% for GG, 45.8% for AG and 16.7% for AA. The frequency distribution of rs17375018 was significantly different from that of Beijing, Utah and Nigeria (P <0.05) There was no difference in Tokyo population distribution (P> 0.05). rs1180530 locus was 24.7% for CC, 47.8% for TC and 27.5% for TT. The frequency distribution of rs1180530 was significantly different from that of Utah, Nigeria (P <0.05), and no significant difference between Beijing and Tokyo > 0.05). Conclusion: The frequency distribution of rs17375018 polymorphism and the frequency distribution of polymorphism in rs11805303 locus in Utah, Utah, Utah, USA are different from those in Utah and Nigeria.